Lytic polysaccharide monooxygenases as powerful tools in enzymatically assisted preparation of nano-scaled cellulose from lignocellulose: A review. (February 2022)

• Record Type: Journal Article
• Authors: Karnaouri, Anthi; Chorozian, Koar; Zouraris, Dimitrios; Karantonis, Antonis; Topakas, Evangelos; Rova, Ulrika; Christakopoulos, Paul
• Is Part Of: Bioresource technology. Volume 345(2022)
• Publication Date: 2022-02
• Subjects: Nanocellulose -- CNFs -- CNCs -- LPMOs -- Enzyme-assisted processes -- Lignocellulose; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.126491
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Lignocellulose-derived nanocellulose is a biocompatible material with excellent properties. C1-oxidative LPMOs introduce –COOH on cellulose surface and promote defibrillation. LPMO-cellulase synergy depends on fiber conformation and enzymes regioselectivities. The successful LPMO-mediated preparation of CNFs and CNCs has been reported. Abstract: Nanocellulose, either in the form of fibers or crystals, constitutes a renewable, biobased, biocompatible material with advantageous mechanical properties that can be isolated from lignocellulosic biomass. Enzyme-assisted isolation of nanocellulose is an attractive, environmentally friendly approach that leads to products of higher quality compared to their chemically prepared counterparts. Lytic polysaccharide monooxygenases (LPMOs) are enzymes that oxidatively cleave the β-1, 4-glycosidic bond of polysaccharides upon activation of O2 or H2 O2 and presence of an electron donor. Their use for treatment of cellulose fibers towards the preparation of nano-scaled cellulose is related to the ability of LPMOs to create nicking points on the fiber surface, thus facilitating fiber disruption and separation. The aim of this review is to describe the mode of action of LPMOs on cellulose fibers towards the isolation of nanostructures, thus highlighting their great potential for the production of nanocellulose as a novel value added product from lignocellulose.