Amorphous Ni(OH)2 nano-boxes as a high performance substrate for aptasensor application. (15th February 2022)

• Record Type: Journal Article
• Authors: Rahmati, Zeinab; Roushani, Mahmoud; Hosseini, Hadi
• Is Part Of: Measurement. Volume 189(2022)
• Publication Date: 2022-02-15
• Subjects: Ni(OH)2 nano-boxes -- Cu2O nanocubes -- Trypsin -- Aptasensor; Weights and measures -- Periodicals; Measurement -- Periodicals; Measurement; Weights and measures; Periodicals; 530.8
• DOI: 10.1016/j.measurement.2021.110649
• Languages: English
• ISSNs: 0263-2241

Abstract

Highlights: The uniform amorphous Ni(OH)2 hollow nano-boxes was fabricated. This multidimensional hollow structure places a large amount of surface area in a small space of electrode surface. It was used for fabricating an advanced electrochemical aptasensor to measure trypsin. The aptasensor show excellent figure of merit to measure trypsin in real samples. Abstract: Recently, the performance of aptasensors has been significantly improved when combined with new nanomaterials. Herein, the amorphous Ni(OH)2 nano-boxes with intact shell structure were synthesized and, then, were used as an efficient substrate for covalent immobilization of the NH2 -functionalized aptamer to develop label-free electrochemical aptasensor ​for ultrasensitive analysis of trypsin. This multidimensional hollow structure places a large amount of surface area in a small space of electrode surface and, thus, increasing the loading of the aptamer strings which significantly increases the sensitivity of ​measurement. A good linear relationship was found between logarithmic value of trypsin and Rct from 1 fg mL −1 to 500 ng mL −1 with a low detection limit of 0.3 fg mL −1 . Furthermore, the proposed aptasensor showed good reproducibility, high stability and excellent feasibility in biological samples. This study showed that the Ni(OH)2 nano-boxes nanocomposite have various advanced electrochemical properties making them suitable electrode materials for aptasensor application.