Determination of the toxicity equivalency factors for ciguatoxins using human sodium channels. (February 2022)

• Record Type: Journal Article
• Authors: Raposo-Garcia, Sandra; Louzao, M. Carmen; Fuwa, Haruhiko; Sasaki, Makoto; Vale, Carmen; Botana, Luis M.
• Is Part Of: Food and chemical toxicology. Volume 160(2022)
• Publication Date: 2022-02
• Subjects: Ciguatoxin -- Gambierol -- Gambierone -- 44-Methyl gambierone -- Sodium channel; CFP ciguatera fish poisoning -- CI Confidence interval -- CTXs ciguatoxins -- DMEM Dulbecco's Modified Eagle Medium -- DMSO Dimethyl sulfoxide -- EFSA European Food Safety Authority -- FDA Food and Drug Administration -- HEK Human embrionic kidney -- ICl chloride current amplitude -- INa sodium current amplitude -- MBA mouse bioassay -- MTX3 maitotoxin 3 -- Pacific CTXs P-CTX -- TEF toxicity equivalency factor -- Vhold holding potential -- VGSC voltage gated sodium channels; Toxicology -- Periodicals; Food poisoning -- Periodicals; Food Poisoning -- Periodicals; Toxicology -- Periodicals; Toxicologie -- Périodiques; Intoxications alimentaires -- Périodiques; Food poisoning; Toxicology; Periodicals; Electronic journals; 615.9
• DOI: 10.1016/j.fct.2022.112812
• Languages: English
• ISSNs: 0278-6915

Abstract

Abstract: Ciguatoxins (CTXs) which are produced by dinoflagellates of the genus Gambierdiscus and Fukuyoa and share a ladder-shaped polyether structure, are causative compounds of one of the most frequent foodborne illness disease known as ciguatera fish poisoning (CFP). CFP was initially found in tropical and subtropical areas but nowadays the dinoflagellates producers of ciguatoxins had spread to European coasts. Therefore, this raises the need of establishing toxicity equivalency factors for the different compounds that can contribute to ciguatera fish poisoning, since biological methods have been replaced by analytical techniques. Thus, in this work, the effects of six compounds causative of ciguatera, on their main target, the human voltage-gated sodium channels have been analyzed for the first time. The results presented here led to the conclusion that the order of potency was CTX1B, CTX3B, CTX4A, gambierol, gambierone and MTX3. Furthermore, the data indicate that the activation voltage of sodium channels is more sensitive to detect ciguatoxins than their effect on the peak sodium current amplitude.