Modification of chitosan nanofibers with CuS and fucoidan for antibacterial and bone tissue engineering applications. (1st April 2022)

• Record Type: Journal Article
• Authors: Lu, Hsien-Tsung; Huang, Guan-Yu; Chang, Wong-Jin; Lu, Tzu-Wei; Huang, Tzu-Wen; Ho, Ming-Hua; Mi, Fwu-Long
• Is Part Of: Carbohydrate polymers. Volume 281(2022)
• Publication Date: 2022-04-01
• Subjects: Chitosan -- Nanofibers -- CuS nanoparticles -- Photothermal -- Photocatalytic -- Antibacterial -- Bone tissue regeneration -- Wound dressing; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.119035
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Chitosan (CS) electrospun nanofiber (ENF) membranes were modified with fucoidan (Fu) and CuS NPs through polyelectrolyte complexation and genipin (GP)-involved cross-linking reaction. The formation of Fu/CS complex and cross-linking of CS with GP increased the acid resistance and reduced the swelling rate of CS ENF, while the covalent conjugation of CuS NPs provided CS ENF with durable Fenton-like catalytic activity. The CuS@ENF composite (ENFC) effectively adsorbed H2 O2 and near-infrared (NIR) light, enabling it to kill bacteria by photothermal and photocatalytic bactericidal effects. Fu and copper ions were able to release from the ENFC in a pH-dependent manner, and promoted the alkaline phosphatase activity of osteoblast cells and capillary tube formation of endothelial cells. This study provides a new approach to modify CS ENF with antibacterial and osteoblast differentiation activities, which may be available for bone infection prevention and tissue regeneration. Graphical abstract: Unlabelled Image Highlights: Modifying chitosan electrospun nanofibers (CS ENFs) with fucoidan (Fu) and CuS NPs Formation of Fu/CS polyelectrolyte complex and covalent conjugation of CuS NPs CuS@ENF composites have chemo-photodynamic and photothermal bactericidal effects. CuS@ENF composites promote the alkaline phosphatase activity of osteoblast cells.