Hsa_circ_0001806 promotes glycolysis and cell progression in hepatocellular carcinoma through miR‐125b/HK2. Issue 12 (19th October 2021)

• Record Type: Journal Article
• Authors: Chen, Xueyi; She, Pengyun; Wang, Caihua; Shi, Lina; Zhang, Tieying; Wang, Yanfei; Li, Haixia; Qian, Lu; Li, Man
• Is Part Of: Journal of clinical laboratory analysis. Volume 35:Issue 12(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-10-19
• Subjects: glycolysis -- hepatocellular carcinoma -- HK2 -- hsa_circ_0001806 -- miR‐125b; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.23991
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract: Objective: Hepatocellular carcinoma (HCC) is one of the most common malignant tumours and a leading cause of cancer death. Circular RNA (circRNA) has been demonstrated to play an important role in regulating tumour development. The current study aims to explore the specific role of hsa_circ_0001806 during HCC progression. Methods: The expression of hsa_circ_0001806 in HCC tissues and cells was measured through qRT‐PCR. Cell proliferation, apoptosis and migration were measured using CCK‐8 and Annexin V/PI staining kits, and Transwell assay. Bioinformatics prediction and dual‐luciferase reporter assay were adopted to explore the mechanism underlying the cell function of hsa_circ_0001806 in HCC cells. In addition, glycolysis was assessed by measuring the glucose uptake, lactate production and ATP level using a glucose assay kit, fluorometric lactate assay kit and ATP detection assay kit. Results: Hsa_circ_0001806 was up‐regulated in HCC tissues and cells and positively associated with the advanced TNM stage, metastasis and poor overall survival. The overexpression of hsa_circ_0001806 promoted HCC cell proliferation, migration and glycolysis and inhibited cell apoptosis, while the silence of hsa_circ_0001806 showed an opposite effect. Furthermore, hsa_circ_0001806 acted as a sponge of miR‐125b to up‐regulate hexokinase II (HK2) expression. In addition, the inhibition of miR‐125b and HK2 overexpression partly reversed the inhibitory effect of hsa_circ_0001806 silencing on HCC cell proliferation, migration and glycolysis. Conclusion: The inhibition of hsa_circ_0001806 suppressed HCC cell proliferation, migration and glycolysis through mediating miR‐125b/HK2 axis. Abstract : Circ_0001806 is up‐regulated in HCC tissues and cells and was positively associated with the advanced TNM stage, metastasis and worse overall survival. Overexpression of circ_0001806 promoted HCC cell proliferation, migration and glycolysis through acting as a sponge of miR‐125b, up‐regulating HK2 expression. Inhibition of miR‐125b or overexpression of HK2 partly reversed the inhibitory effect of circ_0001806 silencing on HCC cell proliferation, migration and glycolysis.