Dihydropyrimidine dehydrogenase (DPYD) gene c.1627A>G A/G and G/G genotypes are risk factors for lymph node metastasis and distant metastasis of colorectal cancer. Issue 11 (6th October 2021)

• Record Type: Journal Article
• Authors: Zeng, Juanzi; Wu, Heming; Huang, Qingyan; Li, Jiaquan; Yu, Zhikang; Zhong, Zhixiong
• Is Part Of: Journal of clinical laboratory analysis. Volume 35:Issue 11(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-10-06
• Subjects: colorectal cancer -- dihydropyrimidine dehydrogenase -- distant metastasis -- DPYD -- lymph node metastasis; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.24023
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract: Background: Dihydropyrimidine dehydrogenase (DPD) acts as the key enzyme catabolizing pyrimidines, and may affect the tumor progression. DPYD gene mutations affect DPD activity. The relationship between DPYD IVS14+1G>A, c.1627A>G, c.85T>C and lymph node metastasis (LNM) and distant metastasis (DM) of colorectal cancer (CRC) was investigated. Methods: A total of 537 CRC patients were enrolled in this study. DPYD polymorphisms were analyzed by polymerase chain reaction (PCR)‐Sanger sequencing. The relationship between DPYD genotypes and clinical features of patients, metastasis of CRC was analyzed. Results: About DPYD c.1627A>G, A/A (57.7%) was the most common genotype, followed by A/G (35.6%), G/G (6.7%) genotypes. In c.85T>C, T/T, T/C, and C/C genotypes are accounted for 83.6%, 16.0%, and 0.4%, respectively. Logistic regression analysis revealed that DPYD c.1627A>G A/G and G/G genotypes in the dominant model (A/G + G/G vs. A/A) were significant risk factors for the LNM ( p = 0.029, OR 1.506, 95% CI = 1.048–2.165) and DM ( p = 0.039, OR 1.588, 95% CI = 1.041–2.423) of CRC. In addition, DPYD c.1627A>G polymorphism was more common in patients with abnormal serum carcinoembryonic antigen (CEA) (>5 ng/ml) ( p = 0.003) or carbohydrate antigen 24–2 (CA24‐2) (>20 U/ml) level ( p = 0.015). Conclusions: The results suggested that DPYD c.1627A>G A/G, G/G genotypes are associated with increased risk of LNM and DM of CRC. Abstract : The association between dihydropyrimidine dehydrogenase (DPYD) gene polymorphisms and lymph node metastasis and distant metastasis of colorectal cancer is not clear. To analyze the relationship between DPYD and metastasis of colorectal cancer among Hakka population in southern China. DPYD gene c.1627A>G A/G and G/G genotypes are risk factors for lymph node metastasis and distant metastasis of colorectal cancer. This study provides valuable information for the prediction of metastatic risk of colorectal cancer and provides a potential target for the clinical treatment of metastatic colorectal cancer.