Associations between inflammasome‐related gene NLRP3 Polymorphisms (rs10754558 and rs35829419) and risk of bladder cancer in a Chinese population. Issue 11 (12th October 2021)

• Record Type: Journal Article
• Authors: Xu, Gang; Huang, Ruohui; Xia, Wei; Jiang, Bo; Xiao, Guancheng; Li, Yanmin
• Is Part Of: Journal of clinical laboratory analysis. Volume 35:Issue 11(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-10-12
• Subjects: bladder cancer -- Chinese population -- NLRP3 -- polymorphism; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.23973
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract: Background: NLRP3 inflammasome as a component of immune system has been found related to several cancers, but no study has assessed NLRP3 polymorphisms on risk of bladder cancer (BC). We aim to investigate whether NLRP3 polymorphisms are associated with the risk and clinical features of bladder cancer (BC) in a Chinese population. Methods: Genotype frequency of two commonly studied NLRP3 SNPs (rs10754558 and rs35829419) was examined in 154 patients with BC and the 308 healthy controls. NLRP3 gene polymorphisms were genotyped by polymerase chain reaction‐restriction fragment length polymorphism method. Results: The distribution frequencies of GG, AG+GG, GG, and G allele in NLRP3 (rs10754558) genotypes were significantly different between case and control group (OR = 2.296, P = .022; OR = 1.598, P = .020; OR = 1.998, P = .049; OR = 1.557, P = .006), but no statistical difference existed for rs35829419. Among smokers and alcohol drinkers, for rs10754558, individuals with AG, GG, and GG+AG genotypes had a higher BC risk compared with individuals with AA; for rs35829419, individuals with variant genotypes (AG and GG+AG) had a stronger risk of developing BC compared with individuals with AA (all P < .05). In stratified analyses of tumor size and tumor node metastasis, AG or GG genotypes of rs10754558 and rs35829419 SNPs were associated with BC risk (both P < .05). Conclusion: NLRP3 polymorphisms (rs10754558 and rs35829419) were related to BC risk and tumor size and lymph node metastasis, especially among smokers and alcohol drinkers. Abstract : The distribution frequencies of AA, AG, and GG genotypes for NLRP3 rs10754558 SNP were significantly different between case and control group, but no statistical difference existed for rs35829419. For rs10754558, compared with those carried AA genotype, the ORs and its 95% CIs were 1.464 (0.960, 2.231) for those carried AG genotype and 2.296 (1.125, 4.685) for those carried GG genotype. For rs35829419, compared with those carried AA genotype, the ORs and its 95% CIs were 1.254 (0.819, 1.918) for those carried AG genotype and 1.679 (0.821, 3.433) for those carried GG genotype.