Evaluation of the automated Becton Dickinson MAX real-time PCR platform for detection of Pneumocystis jirovecii. (January 2017)

• Record Type: Journal Article
• Authors: Chien, Jung-Yien; Liu, Chia-Jung; Chuang, Pei-Chien; Lee, Tai-Fen; Huang, Yu-Tsung; Liao, Chun-Hsing; Hung, Chien-Ching; Sheng, Wan-Huei; Yu, Chong-Jen; Hsueh, Po-Ren
• Is Part Of: Future microbiology. Volume 12:Number 1(2017)
• Page Start: 29
• Page End: 37
• Publication Date: 2017-01
• Subjects: major surface glycoprotein -- MSG -- mtLSU PCR -- nested mitochondrial large subunit (mtLSU) PCR -- PCR -- Pneumocystis jirovecii -- P. jirovecii pneumonia -- real-time PCR; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2016-0115
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: We evaluated the performance of the automated quantitative BD MAX (Becton Dickinson) real-time PCR platform for detecting Pneumocystis jirovecii .Materials & methods: A total of 34 retrospective and 137 prospective samples were included.Results: Retrospectively, all (100%) positive samples were correctly detected by this platform compared with a nested PCR. Among prospective samples, the overall sensitivity, specificity, positive likelihood ratio and negative likelihood ratio were 92.6%, 94.5%, 17.0 and 0.1, respectively. All bronchoalveolar lavage fluid (BALF)/bronchial washing samples were correctly identified by this platform. Samples from patients with colonization had significantly higher median amplification cycle threshold values than patients with P. jirovecii pneumonia.Conclusion: The quantitative BD MAX real-time PCR is a rapid and highly sensitive modality for detecting P. jirovecii, especially in samples from bronchoalveolar lavage fluid/bronchial washing fluid.