Understanding protein glycosylation pathways in bacteria. (January 2017)

• Record Type: Journal Article
• Authors: Li, Hong; Debowski, Aleksandra W; Liao, Tingting; Tang, Hong; Nilsson, Hans-Olof; Marshall, Barry J; Stubbs, Keith A; Benghezal, Mohammed
• Is Part Of: Future microbiology. Volume 12:Number 1(2017)
• Page Start: 59
• Page End: 72
• Publication Date: 2017-01
• Subjects: glycoengineering -- glycosylation pathway -- protein glycosylation; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2016-0166
• Languages: English
• ISSNs: 1746-0913

Abstract

Through advances in analytical methods to detect glycoproteins and to determine glycan structures, there have been increasing reports of protein glycosylation in bacteria. In this review, we summarize the known pathways for bacterial protein glycosylation: lipid carrier-mediated ' en bloc ' glycosylation; and cytoplasmic stepwise protein glycosylation. The exploitation of bacterial protein glycosylation systems, especially the 'mix and match' of three independent but similar pathways (oligosaccharyltransferase-mediated protein glycosylation, lipopolysaccharide and peptidoglycan biosynthesis) in Gram-negative bacteria for glycoengineering recombinant glycoproteins is also discussed.