RpoE promotes invasion and intracellular survival by regulating SPI-1 and SPI-2 in Salmonella enterica serovar Typhi. (August 2016)

• Record Type: Journal Article
• Authors: Zhang, Haifang; Jia, Yanwei; Xie, Xiaofang; Wang, Min; Zheng, Yi; Xu, Shungao; Zhang, Wei; Wang, Qiang; Huang, Xinxiang; Du, Hong
• Is Part Of: Future microbiology. Volume 11:Number 8(2016:Aug.)
• Page Start: 1011
• Page End: 1024
• Publication Date: 2016-08
• Subjects: hyperosmotic stress -- RpoE -- Salmonella enterica serovar Typhi -- SipC -- SPI-1 -- SPI-2; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb.16.19
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: To demonstrate the role of RpoE during the later stage of hyperosmotic stress in Salmonella .Materials & methods: Expressions of SPI-1 and SPI-2 under hyperosmotic stress for 120 min were investigated by a microarray, and the invasion and intracellular survival of wild-type and Δ rpoE strains were compared. The global differential expression of bacterial proteins between the wild-type and Δ rpoE strains was examined after 120 min of hyperosmotic stress.Results: SPI-1 and SPI-2 were repressed, and the invasion and intracellular survival were defected in the Δ rpoE strain. Thirteen bacterial-associated proteins and 11 secreted proteins differed significantly between the wild-type and Δ rpoE strains.Conclusion: RpoE may promote invasion and intracellular survival by regulating the expression of SPI-1 and SPI-2.