Elevated expression of histone demethylase PHF8 associates with adverse prognosis in patients of laryngeal and hypopharyngeal squamous cell carcinoma. (April 2015)

• Record Type: Journal Article
• Authors: Zhu, Gangcai; Liu, Lijun; She, Li; Tan, Haolei; Wei, Ming; Chen, Changhan; Su, Zhongwu; Huang, Donghai; Tian, Yongquan; Qiu, Yuanzheng; Liu, Yong; Zhang, Xin
• Is Part Of: Epigenomics. Volume 7:Number 2(2015)
• Page Start: 143
• Page End: 153
• Publication Date: 2015-04
• Subjects: epigenetic marker -- H3K9 -- H3K27 -- histone demethylation -- laryngeal and hypopharyngeal squamous cell carcinoma -- metastasis -- PHF8 -- recurrence -- squamous cell carcinoma of the head and neck -- survival time; Epigenesis -- Periodicals; Genomics -- Periodicals; Chromatin -- Periodicals; DNA -- Periodicals; 572.87
• DOI: 10.2217/epi.14.82
• Languages: English
• ISSNs: 1750-1911

Abstract

Aim: Overexpression of histone demethylase PHF8 has been reported to function as an oncoprotein in many cancers; however, the implications of PHF8 involvement in laryngeal and hypopharyngeal squamous cell carcinoma (LHSCC) remain unclear. This study aims to explore the expression of PHF8 and its clinical significance in LHSCC.Materials & methods: Western blotting and immunohistochemistry were performed to evaluate PHF8 protein expression in fresh and archived LHSCC samples. Global expressions of H3K27 and H3K9 methylation were analyzed in a cell line with PHF8 siRNA treatment.Results & conclusion: In our study, PHF8 was upregulated in fresh LHSCC tissues. Immunohistochemical staining revealed that the expression of PHF8 was positively associated with T classification, clinical stage, primary tumor position and tumor relapse. Survival analysis demonstrated that high PHF8 expression was significantly associated with shorter overall survival and disease-free survival. Moreover, PHF8 regulates the levels of H3K9me2 and H3K27me2 in LHSCC. Taken together, PHF8 might be a novel prognostic marker for this disease.