EndoSd: an IgG glycan hydrolyzing enzyme in Streptococcus dysgalactiae subspecies dysgalactiae. (June 2016)

• Record Type: Journal Article
• Authors: Shadnezhad, Azadeh; Naegeli, Andreas; Sjögren, Jonathan; Adamczyk, Barbara; Leo, Fredrik; Allhorn, Maria; Karlsson, Niclas G; Jensen, Anders; Collin, Mattias
• Is Part Of: Future microbiology. Volume 11:Number 6(2016:Jun.)
• Page Start: 721
• Page End: 736
• Publication Date: 2016-06
• Subjects: asparagine linked -- chitinases -- Endo-β-N-acetylglucosaminidase -- glycosidase -- IgG -- Streptococcus dysgalactiae; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb.16.14
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: The aim of this study was to identify and characterize EndoS-like enzymes in Streptococcus dysgalactiae subspecies dysgalactiae (SDSD).Materials & methods: PCR, DNA sequencing, recombinant protein expression, lectin blot, ultra high performance liquid chromatography analysis and a chitinase assay were used to identify ndoS -like genes and characterize EndoSd.Results: EndoSd were found in four SDSD strains. EndoSd hydrolyzes the chitobiose core of the glycan on IgG. The amino acid sequence of EndoSd is 70% identical to EndoS in S. pyogenes, but it has a unique C-terminal sequence. EndoSd secretion is influenced by the carbohydrate composition of the growth medium.Conclusion: Our findings indicate that IgG glycan hydrolyzing activity is present in SDSD, and that the activity can be attributed to the here identified enzyme EndoSd.