Direct detection of Helicobacter pylori in biopsy specimens using a high-throughput multiple genetic detection system. (December 2016)

• Record Type: Journal Article
• Authors: Zhang, Yanmei; Wang, Shiwen; Hu, Binjie; Zhao, Fuju; Xiang, Ping; Ji, Danian; Chen, Fei; Liu, Xiaoli; Yang, Feng; Wu, Yong; Kong, Mimi; Nan, Li; Miao, Yingxin; Jiang, Wenrong; Fang, Yi; Zhang, Jinghao; Bao, Zhijun; Olszewski, Michal A; Zhao, Hu
• Is Part Of: Future microbiology. Volume 11:Number 12(2016:Dec.)
• Page Start: 1521
• Page End: 1534
• Publication Date: 2016-12
• Subjects: direct high-throughput multiple genetic detection system -- gastric biopsy specimens -- Helicobacter pylori -- identification -- mixed infection -- quantitative analysis -- resistance -- virulence; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2016-0149
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: We evaluated the direct high-throughput multiple genetic detection system (dHMGS) for Helicobacter pylori in gastric biopsies.Materials & methods: One hundred and thirty-three specimens were concurrently analyzed by dHMGS, rapid urease test, culture and sequencing.Results: dHMGS was highly sensitive and specific for H. pylori identification compared with culture and rapid urease test. The correlation coefficient of the quantitative standard curve was R 2 = 0.983. A significant difference in the relative H. pylori DNA abundance was found in different gastroduodenal diseases. Concordance rates between dHMGS and sequencing for resistance mutations were 97.1, 100.0, 85.3 and 97.1%, respectively. Finally, dHMGS could efficiently distinguish mixed infection in biopsy specimens.Conclusion: The dHMGS could efficiently diagnose and quantify H. pylori burden in biopsies, simultaneously screening for virulence, antibiotic resistance and presence of the multistrain infections.