Caveolin-1-mediated Japanese encephalitis virus entry requires a two-step regulation of actin reorganization. (October 2016)

• Record Type: Journal Article
• Authors: Xu, Qingqiang; Cao, Mingmei; Song, Hongyuan; Chen, Shenglin; Qian, Xijing; Zhao, Ping; Ren, Hao; Tang, Hailin; Wang, Yan; Wei, Youheng; Zhu, Yongzhe; Qi, Zhongtian
• Is Part Of: Future microbiology. Volume 11:Number 10(2016:Oct.)
• Page Start: 1227
• Page End: 1248
• Publication Date: 2016-10
• Subjects: actin regulation -- Caveolin-1 -- EGFR -- endocytosis -- Japanese encephalitis virus -- neuronal cells; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2016-0002
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: To investigate the detailed mechanism of Japanese encephalitis virus (JEV) cell entry.Materials & methods: Utilize a siRNA library targeting cellular membrane trafficking genes to identify key molecules that mediate JEV entry into human neuronal cells.Results: JEV enters human neuronal cells by caveolin-1-mediated endocytosis, which depends on a two-step regulation of actin cytoskeleton remodeling triggered by RhoA and Rac1: RhoA activation promoted the phosphorylation of caveolin-1, and then Rac1 activation facilitated caveolin-associated viral internalization. Specifically, virus attachment activates the EGFR–PI3K signaling pathway, thereby leading to RhoA activation.Conclusion: This work provides a detailed picture of the entry route and intricate cellular events following the entry of JEV into human neuronal cells, and promotes a better understanding of JEV entry.