Nanoparticle-loaded microcapsules providing effective UV protection for Cry1Ac. (17th November 2021)

• Record Type: Journal Article
• Authors: Zhang, Yongjing; Zhang, Aijing; Li, Mengyuan; He, Kanglai; Guo, Shuyuan
• Is Part Of: Journal of microencapsulation. Volume 38:Number 7/8(2021)
• Page Start: 522
• Page End: 532
• Publication Date: 2021-11-17
• Subjects: Bacillus thuringiensis -- microcapsule -- nanoparticles -- UV resistance -- insecticidal activity; Microencapsulation -- Periodicals; Controlled release technology -- Periodicals; 615.19
• DOI: 10.1080/02652048.2021.1990424
• Languages: English
• ISSNs: 0265-2048

Abstract

Abstract: Aim: To prepare several novel microcapsules using chitosan (Cs) and Alginate (Alg) as coating materials, and nano-ZnO, nano-SiO2, nano-TiO2 as UV protective agents for improving UV resistance of Cry1Ac. Methods: Microcapsules were prepared by the layer-by-layer (LbL) self-assembly technique and electrostatic adsorption. The morphologies were observed by scanning electron microscopy (SEM), and the stability under UV radiation was studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and bioassay. Results: SEM showed that nano-ZnO and nano-TiO2 could be adsorbed on the negatively charged MC with the outermost layer being Alg, while nano-SiO2 could be adsorbed on the positively charged MC with Cs as the outermost layer. SDS-PAGE and bioassay showed that nano-ZnO and nano-SiO2 could provide effective UV protection after 8 h UV irradiation ( p > 0.05), and nano-TiO2 could provide effective UV protection after 4 h UV irradiation ( p > 0.05). Conclusion: The microcapsules loaded with nanoparticles provided excellent UV resistance for Cry1Ac.