Tobacco smoking-associated genome-wide DNA methylation changes in the EPIC study. (May 2016)

• Record Type: Journal Article
• Authors: Ambatipudi, Srikant; Cuenin, Cyrille; Hernandez-Vargas, Hector; Ghantous, Akram; Le Calvez-Kelm, Florence; Kaaks, Rudolf; Barrdahl, Myrto; Boeing, Heiner; Aleksandrova, Krasimira; Trichopoulou, Antonia; Lagiou, Pagona; Naska, Androniki; Palli, Domenico; Krogh, Vittorio; Polidoro, Silvia; Tumino, Rosario; Panico, Salvatore; Bueno-de-Mesquita, Bas; Peeters, Petra HM; Quirós, José Ramón; Navarro, Carmen; Ardanaz, Eva; Dorronsoro, Miren; Key, Tim; Vineis, Paolo; Murphy, Neil; Riboli, Elio; Romieu, Isabelle; Herceg, Zdenko
• Is Part Of: Epigenomics. Volume 8:Number 5(2016)
• Page Start: 599
• Page End: 618
• Publication Date: 2016-05
• Subjects: DNA methylome -- epigenetic signature -- prospective cohort -- tobacco smoking; Epigenesis -- Periodicals; Genomics -- Periodicals; Chromatin -- Periodicals; DNA -- Periodicals; 572.87
• DOI: 10.2217/epi-2016-0001
• Languages: English
• ISSNs: 1750-1911

Abstract

Aim : Epigenetic changes may occur in response to environmental stressors, and an altered epigenome pattern may represent a stable signature of environmental exposure.Materials & methods: Here, we examined the potential of DNA methylation changes in 910 prediagnostic peripheral blood samples as a marker of exposure to tobacco smoke in a large multinational cohort.Results : We identified 748 CpG sites that were differentially methylated between smokers and nonsmokers, among which we identified novel regionally clustered CpGs associated with active smoking. Importantly, we found a marked reversibility of methylation changes after smoking cessation, although specific genes remained differentially methylated up to 22 years after cessation.Conclusion : Our study has comprehensively cataloged the smoking-associated DNA methylation alterations and showed that these alterations are reversible after smoking cessation.