Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes. (December 2016)

• Record Type: Journal Article
• Authors: Broholm, Christa; Olsson, Anders Henrik; Perfilyev, Alexander; Gillberg, Linn; Hansen, Ninna Schiøler; Ali, Ashfaq; Mortensen, Brynjulf; Ling, Charlotte; Vaag, Allan
• Is Part Of: Epigenomics. Volume 8:Number 12(2016)
• Page Start: 1601
• Page End: 1617
• Publication Date: 2016-12
• Subjects: adipogenesis -- adiponectin -- DNA methylation -- epigenetics -- stem cells; Epigenesis -- Periodicals; Genomics -- Periodicals; Chromatin -- Periodicals; DNA -- Periodicals; 572.87
• DOI: 10.2217/epi-2016-0077
• Languages: English
• ISSNs: 1750-1911

Abstract

Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis.Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes.Results: We observed altered DNA methylation of 11, 947 CpG sites and altered expression of 11, 830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling.Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.