The methylome and transcriptome of fetal skin: implications for scarless healing. (October 2016)

• Record Type: Journal Article
• Authors: Podolak-Popinigis, Justyna; Ronowicz, Anna; Dmochowska, Monika; Jakubiak, Agnieszka; Sachadyn, Paweł
• Is Part Of: Epigenomics. Volume 8:Number 10(2016)
• Page Start: 1331
• Page End: 1345
• Publication Date: 2016-10
• Subjects: fetal skin -- genome-wide DNA methylation profiling -- genome-wide gene expression profiling -- scarless skin wound healing; Epigenesis -- Periodicals; Genomics -- Periodicals; Chromatin -- Periodicals; DNA -- Periodicals; 572.87
• DOI: 10.2217/epi-2016-0068
• Languages: English
• ISSNs: 1750-1911

Abstract

Aim: Fetal skin is known to heal without scarring. In mice, the phenomenon is observed until the 16–17 day of gestation – the day of transition from scarless to normal healing. The study aims to identify key methylome and transcriptome changes following the transition.Materials & methods: Methylome and transcriptome profiles were analyzed in murine dorsal skin using microarray approach.Results & conclusion: The genes associated with inflammatory response and hyaluronate degradation showed increased DNA methylation before the transition, while those involved in embryonic morphogenesis, neuron differentiation and synapse functions did so after. A number of the methylome alterations were retained until adulthood and correlated with gene expression, while the functional associations imply that scarless healing depends on epigenetic regulation.