Delaying or delivering: identification of novel NAM-1 alleles that delay senescence to extend wheat grain fill duration. (18th August 2021)

• Record Type: Journal Article
• Authors: Chapman, Elizabeth A; Orford, Simon; Lage, Jacob; Griffiths, Simon
• Editors: Rebetzke, Greg
• Is Part Of: Journal of experimental botany. Volume 72:Number 22(2021)
• Page Start: 7710
• Page End: 7728
• Publication Date: 2021-08-18
• Subjects: Bulk segregant analysis -- exome capture -- forward genetics -- grain fill -- mutant -- NAM -- remobilization -- senescence -- staygreen -- wheat; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/erab368
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract: Senescence is a complex trait under genetic and environmental control, in which resources are remobilized from vegetative tissue into grain. Delayed senescence, or 'staygreen' traits, can confer stress tolerance, with extended photosynthetic activity hypothetically sustaining grain filling. The genetics of senescence regulation are largely unknown, with senescence variation often correlated with phenological traits. Here, we confirm staygreen phenotypes of two Triticum aestivum cv. Paragon ethyl methane sulfonate mutants previously identified during a forward genetic screen and selected for their agronomic performance, similar phenology, and differential senescence phenotypes. Grain filling experiments confirmed a positive relationship between onset of senescence and grain fill duration, reporting an associated ~14% increase in final dry grain weight for one mutant ( P <0.05). Recombinant inbred line (RIL) populations segregating for the timing of senescence were developed for trait mapping purposes and phenotyped over multiple years under field conditions. Quantification and comparison of senescence metrics aided RIL selection, facilitating exome capture-enabled bulk segregant analysis (BSA). Using BSA we mapped our two staygreen traits to two independent, dominant, loci of 4.8 and 16.7 Mb in size encompassing 56 and 142 genes, respectively. Combining association analysis with variant effect prediction, we identified single nucleotide polymorphisms encoding self-validating mutations located in NAM-1 homoeologues, which we propose as gene candidates. Abstract : Two staygreen mutants were identified for which grain fill duration and grain weight were enhanced. Independent amino acid substitutions within NAC subdomain IV of NAM-1 homoeologues are proposed as causative.