Chromatin accessibility landscapes activated by cell-surface and intracellular immune receptors. (13th August 2021)

• Record Type: Journal Article
• Authors: Ding, Pingtao; Sakai, Toshiyuki; Krishna Shrestha, Ram; Manosalva Perez, Nicolas; Guo, Wenbin; Ngou, Bruno Pok Man; He, Shengbo; Liu, Chang; Feng, Xiaoqi; Zhang, Runxuan; Vandepoele, Klaas; MacLean, Dan; Jones, Jonathan D G
• Editors: Lunn, John
• Is Part Of: Journal of experimental botany. Volume 72:Number 22(2021)
• Page Start: 7927
• Page End: 7941
• Publication Date: 2021-08-13
• Subjects: ATAC-seq and RNA-seq -- cell surface immune receptors -- chromatin accessibility -- gene regulatory network -- intracellular immune receptors -- plant innate immunity -- PRR and NLR -- transcriptional regulation; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/erab373
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract : Effective plant immunity requires activation of both cell-surface and intracellular receptors, which increases chromatin accessibility at induced defence genes, under the control of both conserved and unique transcriptional regulatory mechanisms. Abstract: Activation of cell-surface and intracellular receptor-mediated immunity results in rapid transcriptional reprogramming that underpins disease resistance. However, the mechanisms by which co-activation of both immune systems lead to transcriptional changes are not clear. Here, we combine RNA-seq and ATAC-seq to define changes in gene expression and chromatin accessibility. Activation of cell-surface or intracellular receptor-mediated immunity, or both, increases chromatin accessibility at induced defence genes. Analysis of ATAC-seq and RNA-seq data combined with publicly available information on transcription factor DNA-binding motifs enabled comparison of individual gene regulatory networks activated by cell-surface or intracellular receptor-mediated immunity, or by both. These results and analyses reveal overlapping and conserved transcriptional regulatory mechanisms between the two immune systems.