An early developmental vertebrate model for nanomaterial safety: bridging cell-based and mammalian toxicity assessment. (March 2016)

• Record Type: Journal Article
• Authors: Webster, Carl A; Di Silvio, Desire; Devarajan, Aarthi; Bigini, Paolo; Micotti, Edoardo; Giudice, Chiara; Salmona, Mario; Wheeler, Grant N; Sherwood, Victoria; Bombelli, Francesca Baldelli
• Is Part Of: Nanomedicine. Volume 11:Number 6(2016)
• Page Start: 643
• Page End: 656
• Publication Date: 2016-03
• Subjects: cytotoxicity -- in vitro–in vivo correlations -- nanomaterials -- nanomedicine -- nanotoxicity -- Xenopus laevis; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.15.219
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: With the rise in production of nanoparticles (NPs) for an ever-increasing number of applications, there is an urgent need to efficiently assess their potential toxicity. We propose a NP hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring to determine an overall toxicity index.Results: We observed that, after exposure to a range of NPs, Xenopus phenotypic scoring showed a strong correlation with cell based in vitro assays. Magnetite-cored NPs, negative for toxicity in vitro and Xenopus, were further confirmed as nontoxic in mice.Conclusion: The results highlight the potential of Xenopus embryo analysis as a fast screening approach for toxicity assessment of NPs, which could be introduced for the routine testing of nanomaterials.