Preparation and immunogenicity of gold glyco-nanoparticles as antipneumococcal vaccine model. (January 2017)

• Record Type: Journal Article
• Authors: Vetro, Maria; Safari, Dodi; Fallarini, Silvia; Salsabila, Korrie; Lahmann, Martina; Penadés, Soledad; Lay, Luigi; Marradi, Marco; Compostella, Federica
• Is Part Of: Nanomedicine. Volume 12:Number 1(2017)
• Page Start: 13
• Page End: 23
• Publication Date: 2017-01
• Subjects: capsular polysaccharide fragments -- carbohydrate-based vaccines -- gold glyco-nanoparticles -- immunogenicity -- Streptococcus pneumoniae; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2016-0306
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: Nanotechnology-based fully synthetic carbohydrate vaccines are promising alternatives to classic polysaccharide/protein conjugate vaccines. We have prepared gold glyco-nanoparticles (GNP) bearing two synthetic carbohydrate antigens related to serotypes 19F and 14 of Streptococcus pneumoniae and evaluated their immunogenicity in vivo .Results: A tetrasaccharide fragment of serotype 14 (Tetra-14), a trisaccharide fragment of serotype 19F (Tri-19F), a T-helper peptide andd -glucose were loaded onto GNP in different ratios. Mice immunization showed that the concomitant presence of Tri-19F and Tetra-14 on the same nanoparticle critically enhanced the titers of specific IgG antibodies toward type 14 polysaccharide compared with GNP exclusively displaying Tetra-14, while no IgG antibodies against type 19F polysaccharide were elicited.Conclusion: This work is a step forward toward synthetic nanosystems combining carbohydrate antigens and immunogenic peptides as potential carbohydrate-based vaccines. Graphical abstract: