Double adjuvanting strategy for peptide-based vaccines: trimethyl chitosan nanoparticles for lipopeptide delivery. (December 2016)

• Record Type: Journal Article
• Authors: Marasini, Nirmal; Giddam, Ashwini K; Khalil, Zeinab G; Hussein, Waleed M; Capon, Robert J; Batzloff, Michael R; Good, Michael F; Toth, Istvan; Skwarczynski, Mariusz
• Is Part Of: Nanomedicine. Volume 11:Number 24(2016)
• Page Start: 3223
• Page End: 3235
• Publication Date: 2016-12
• Subjects: chitosan -- dextran -- Group A Streptococcus -- lipopeptides -- nasal -- vaccine; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2016-0291
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: To develop novel polymer-based nanoscale delivery system for lipopeptide-based vaccine against group A Streptococcus (GAS).Materials & methods: Four types of lipopeptide antigen-loaded polymeric nanoparticles (NP) were prepared. NP were accessed for their capacity to be taken up by dendritic cells; effect on dendritic cell maturation; ability to induce mucosal and systemic immunity; and capability to induce antibody responses that opsonize GAS bacteria.Results & discussion: The combination of adjuvanting properties of lipopeptides and dextran/trimethyl chitosan-based NP had a synergistic effect on humoral immunity, and the produced antibodies showed high opsonic activity against clinical GAS isolates.Conclusion: Biocompatible NP-bearing trimethyl chitosan and dextran are efficient as mucosal adjuvants for the intranasal delivery of lipopeptide-based vaccines.