Characterization of ferucarbotran-loaded RBCs as long circulating magnetic contrast agents. (November 2016)

• Record Type: Journal Article
• Authors: Antonelli, Antonella; Sfara, Carla; Weber, Oliver; Pison, Ulrich; Manuali, Elisabetta; Salamida, Sonia; Magnani, Mauro
• Is Part Of: Nanomedicine. Volume 11:Number 21(2016)
• Page Start: 2781
• Page End: 2795
• Publication Date: 2016-11
• Subjects: ferucarbotran -- magnetic particle imaging -- magnetic particle spectroscopy -- magnetic resonance imaging -- red blood cells -- superparamagnetic iron oxides; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2016-0216
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: The biomedical application of contrast agents based on superparamagnetic iron oxide nanoparticles is still limited because of their short intravascular half-life. The potential of red blood cells (RBCs) loaded with new ferucarbotran nanoparticles as magnetic contrast agents with longer blood retention time has been investigated.Materials & methods: Ferucarbotran was loaded into RBCs by a procedure of hypotonic dialysis and isotonic resealing. Ferucarbotran amounts encapsulated in RBCs were determined by NMR. The survival of ferucarbotran-loaded RBCs and bulk ferucarbotran was evaluated in the mouse bloodstream.Results: Blood retention time of these RBC constructs is longer (∼14 days) than the bulk ferucarbotran (∼1 h) with a slower Fe clearance from liver and spleen.Conclusion: Ferucarbotran-loaded RBCs could be used as potential contrasting agents for diagnostic applications in MRI/magnetic particle imaging.