Active diffusion of nanoparticles of maternal origin within the embryonic brain. (October 2016)

• Record Type: Journal Article
• Authors: Yuryev, Mikhail; Ferreira, Mónica PA; Balasubramanian, Vimalkumar; Correia, Alexandra MR; Mäkilä, Ermei M; Jokinen, Ville; Andriichuk, Liliia; Kemell, Marianna; Salonen, Jarno J; Hirvonen, Jouni T; Santos, Hélder A; Rivera, Claudio
• Is Part Of: Nanomedicine. Volume 11:Number 19(2016)
• Page Start: 2471
• Page End: 2481
• Publication Date: 2016-10
• Subjects: development -- embryos -- mother–embryo interface -- nanoparticles -- porous silicon; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2016-0207
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: To investigate porous silicon (PSi) nanoparticles (NPs) behavior in the embryonic brain.Materials & methods: Fluorescently labeled PSi NPs were injected into the embryonic brains intraventricularly and to the mother intravenously (iv.). Brain histology from different time points up to 3 days was analyzed and live brains imaged with two-photon microscopy.Results: PSi NPs were able to penetrate 80% of the embryonic cortical depth. Particle motility was confirmed in real-time in vivo . PSi NPs were able to penetrate the embryonic cortex after either iv. maternal or intraventricular injection. No developmental of macromorphological changes or increased cell apoptosis was observed.Conclusion: PSi NPs penetrate deep in the brain tissues of embryos after intraventricular injection and after iv. injection to the mother.