CXCR4+-targeted protein nanoparticles produced in the food-grade bacterium Lactococcus lactis. (September 2016)

• Record Type: Journal Article
• Authors: Cano-Garrido, Olivia; Céspedes, María Virtudes; Unzueta, Ugutz; Saccardo, Paolo; Roldán, Mònica; Sánchez-Chardi, Alejandro; Cubarsi, Rafael; Vázquez, Esther; Mangues, Ramon; García-Fruitós, Elena; Villaverde, Antonio
• Is Part Of: Nanomedicine. Volume 11:Number 18(2016)
• Page Start: 2387
• Page End: 2398
• Publication Date: 2016-09
• Subjects: protein nanoparticles -- recombinant proteins -- tumor-homing peptides; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2016-0200
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: Lactococcus lactis is a Gram-positive (endotoxin-free) food-grade bacteria exploited as alternative to Escherichia coli for recombinant protein production. We have explored here for the first time the ability of this platform as producer of complex, self-assembling protein materials.Materials & methods: Biophysical properties, cell penetrability and in vivo biodistribution upon systemic administration of tumor-targeted protein nanoparticles produced in L. lactis have been compared with the equivalent material produced in E. coli .Results: Protein nanoparticles have been efficiently produced in L. lactis, showing the desired size, internalization properties and biodistribution.Conclusion: In vitro and in vivo data confirm the potential and robustness of the production platform, pointing out L. lactis as a fascinating cell factory for the biofabrication of protein materials intended for therapeutic applications.