Aggregation and protein corona formation on gold nanoparticles affect viability and liver functions of primary rat hepatocytes. (September 2016)

• Record Type: Journal Article
• Authors: Koh, Wee Ling; Tham, Phoebe Huijun; Yu, Hanry; Leo, Hwa Liang; Yong Kah, James Chen
• Is Part Of: Nanomedicine. Volume 11:Number 17(2016)
• Page Start: 2275
• Page End: 2287
• Publication Date: 2016-09
• Subjects: gold nanoparticles -- hepatotoxicity -- liver toxicology -- primary rat hepatocytes; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2016-0173
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: We examined the impact of aggregation and protein corona formation of gold nanoparticles (AuNPs) on the cytotoxicity, uptake and metabolism, specifically urea and albumin synthesis, of primary rat hepatocytes.Materials & methods: The AuNPs were synthesized via citrate reduction and the human serum protein corona was preformed on the AuNPs. Primary hepatocytes were isolated from male Wistar rats via two-step in situ collagenase perfusion method, and were dosed with both citrate-capped (AuNP-Cit) and protein corona coated AuNPs (AuNP-Cor).Results: The AuNP-Cor showed higher cell uptake and reduced cell viability compared with aggregated AuNP-Cit. Urea and albumin secretions showed AuNP dose dependency. Both AuNP-Cit and AuNP-Cor exerted only an acute effect on the albumin synthesis of hepatocytes with no chronic impact.