Surface modification of SOI-FET sensors for label-free and specific detection of short RNA analyte. (August 2016)

• Record Type: Journal Article
• Authors: Dmitrienko, Elena; Naumova, Olga; Fomin, Boris; Kupryushkin, Maxim; Volkova, Alena; Amirkhanov, Nariman; Semenov, Dmitry; Pyshnaya, Inna; Pyshnyi, Dmitrii
• Is Part Of: Nanomedicine. Volume 11:Number 16(2016)
• Page Start: 2073
• Page End: 2082
• Publication Date: 2016-08
• Subjects: short RNA analyte -- SOI-FET biosensor -- surface modification; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2016-0071
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: A new type of surface modification of SOI-FET sensors with ultrathin sensor-probe transition layer and uncharged probes for highly specific detection of short RNA (saRNA) was suggested.Materials & methods: Carbonyldiimidazole (CDI) or glycidoxypropyltrimethoxysilane were used as precursors of sensor-probe interface layers, together with peptide nucleic acids and new NA analogues – phosphoryl guanidine oligo(2′-OMe)ribonucleotides (PGO) as probes for RNA hybridization. RNA sequences corresponding to mRNA NELFA (NM_005663) and microRNA-29a (cancer markers) were used as saRNA targets. Real-time saRNA detection by SOI-FET sensors and fluorescence analysis were applied.Results: Highly specific response with femtomolar sensitivity to saRNA was demonstrated for CDI-PGO-modified sensors fabricated by optical lithography.Conclusion: The proposed CDI–PGO protocol of modification of Si sensor surface is a promising procedure for biomedical applications.