Electrochemical sensing of biomarker for diagnostics of bacteria-specific infections. (August 2016)

• Record Type: Journal Article
• Authors: Alatraktchi, Fatima AlZahra'a; Johansen, Helle Krogh; Molin, Søren; Svendsen, Winnie Edith
• Is Part Of: Nanomedicine. Volume 11:Number 16(2016)
• Page Start: 2185
• Page End: 2195
• Publication Date: 2016-08
• Subjects: amperometry -- bacteria -- biosensors -- cystic fibrosis -- detection -- diagnosis -- electrochemistry -- electrodes -- Pseudomonas aeruginosa -- pyocyanin; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2016-0155
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: Pseudomonas aeruginosa is a pathogen that is prevalent in serious infections in compromised patients worldwide. A unique virulence factor of this bacterium is the redox-active molecule pyocyanin, which is a potential biomarker for the identification of P. aeruginosa infections. Here we report a direct, selective and rapid detection technique of pyocyanin.Materials & methods: Pyocyanin was detected by amperometry at a relatively high potential where the pyocyanin signal was unaffected by background contributions.Results & conclusion: Pyocyanin was detected at concentrations down to 125 nM in a 50 µM mixture of interfering compounds with a reproducibility of r 2 = 0.999 (n = 5) within 200 s. The results document a step toward a point-of-care technique for diagnosis of P. aeruginosa infections.