Surviving nebulization-induced stress: dexamethasone in pH-sensitive archaeosomes. (August 2016)

• Record Type: Journal Article
• Authors: Altube, Maria Julia; Selzer, Solange Mailen; de Farias, Marcelo Alexandre; Portugal, Rodrigo Villares; Morilla, Maria Jose; Romero, Eder Lilia
• Is Part Of: Nanomedicine. Volume 11:Number 16(2016)
• Page Start: 2103
• Page End: 2117
• Publication Date: 2016-08
• Subjects: lung -- macrophages targeting -- nebulization -- subcellular delivery; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2016-0165
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: To increase the subcellular delivery of dexamethasone phosphate (DP) and stability to nebulization stress, pH-sensitive nanoliposomes (LpH) exhibiting archaeolipids, acting as ligands for scavenger receptors (pH-sensitive archaeosomes [ApH]), were prepared.Materials & methods: The anti-inflammatory effect of 0.18 mg DP/mg total lipid, 100–150 nm DP-containing ApH (dioleylphosphatidylethanolamine: Halorubrum tebenquichense total polar archaeolipids:cholesteryl hemisuccinate 4.2:2.8:3 w:w) was tested on different cell lines. Size and HPTS retention of ApH and conventional LpH (dioleylphosphatidylethanolamine:cholesteryl hemisuccinate 7:3 w:w) before and after nebulization were determined.Results & conclusion: DP-ApH suppressed IL-6 and TNF-α on phagocytic cells. Nebulized after 6-month storage, LpH increased size and completely lost its HPTS while ApH3 conserved size and polydispersity, fully retaining its original HPTS content.