Capturing protein-coding genes across highly divergent species. (June 2013)

• Record Type: Journal Article
• Authors: Li, Chenhong; Hofreiter, Michael; Straube, Nicolas; Corrigan, Shannon; Naylor, Gavin J.P.
• Is Part Of: Biotechniques. Volume 54:Number 6(2013)
• Page Start: 321
• Page End: 326
• Publication Date: 2013-06
• Subjects: comparative biology -- cross-species capture -- DNA hybridization capture -- next-generation sequencing -- protein-coding genes -- phylogenetics -- target enrichment; Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000114039
• Languages: English
• ISSNs: 0736-6205

Abstract

DNA hybridization capture combined with next generation sequencing can be used to determine the sequences of hundreds of target genes across hundreds of individuals in a single experiment. However, the approach has thus far only been successfully applied to capture targets that are highly similar in sequence to the bait molecules. Here we introduce modifications that extend the reach of the method to allow efficient capture of highly divergent homologous target sequences using a single set of baits. These modifications have important implications for comparative biology.