Single step BP/LR combined Gateway reactions. (November 2013)

• Record Type: Journal Article
• Authors: Liang, Xiquan; Peng, Lansha; Baek, Chang-Ho; Katzen, Federico
• Is Part Of: Biotechniques. Volume 55:Number 5(2013)
• Page Start: 265
• Page End: 268
• Publication Date: 2013-11
• Subjects: recombination -- cloning -- clonase -- one-pot reaction; Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000114101
• Languages: English
• ISSNs: 0736-6205

Abstract

The Gateway recombination system is characterized by its ability to transfer DNA sequences back and forth between an intermediate clone (the entry clone) and a variety of destination vectors. However, a number of applications do not need to exploit the advantages offered by the entry clone. Here we report reaction conditions for cloning DNA fragments into destination vectors in a single step reaction, thus reducing the cost and overall time needed to obtain an expression clone from three days to one.