Isolation of segmented filamentous bacteria from complex gut microbiota. (August 2015)

• Record Type: Journal Article
• Authors: Ericsson, Aaron C.; Turner, Giedre; Montoya, Lisa; Wolfe, Annie; Meeker, Stacey; Hsu, Charlie; Maggio-Price, Lillian; Franklin, Craig L.
• Is Part Of: Biotechniques. Volume 59:Number 2(2015)
• Page Start: 94
• Page End: 98
• Publication Date: 2015-08
• Subjects: segmented filamentous bacteria -- SFB -- Candidatus Arthromitus -- culture -- microbiota -- commensal; Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000114319
• Languages: English
• ISSNs: 0736-6205

Abstract

Segmented filamentous bacteria (SFB) modulate the ontogeny of the immune system, and their presence can significantly affect mouse models of disease. Until recently, the inability to successfully culture SFB has made controlled studies on the mechanisms by which these bacteria exert their influence problematic. Here, we report a new method for selecting SFB from complex microbial mixtures, providing researchers a simple and cost-effective means to prepare pure infective inocula for prospective studies and also to compare individual SFB isolates.