Membrane display and functional analysis of juxtacrine ligand-receptor signaling. (October 2015)

• Record Type: Journal Article
• Authors: Nair, Pradeep M.; Ngu, Hai; Torres, Eric; Marsters, Scot; Lawrence, David A.; Stephan, Jean-Philippe; Komuves, Laszlo; Ashkenazi, Avi
• Is Part Of: Biotechniques. Volume 59:Number 4(2015)
• Page Start: 231
• Page End: 240
• Publication Date: 2015-10
• Subjects: transport -- screen -- juxtacrine -- supported membrane display -- caspase -- apoptosis -- death receptor; Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000114342
• Languages: English
• ISSNs: 0736-6205

Abstract

We developed a strategy for identifying modulators of juxtacrine signaling, triggered by a cell-surface ligand displayed on synthetic lipid bilayers, via cognate receptors on apposed cells. Using readouts for receptor lateral transport and intracellular signaling, we screened a small interfering RNA (siRNA) library and identified specific receptor tyrosine kinases (RTKs) that directly or indirectly modulate apoptosis signaling by a model death ligand through its cognate death receptors. This approach may be broadly useful for studying juxtacrine cell–cell signaling systems.