Single-step PCR-based genetic sex determination of rat tissues and cells. (May 2017)

• Record Type: Journal Article
• Authors: Dhakal, Pramod; Soares, Michael J.
• Is Part Of: Biotechniques. Volume 62:Number 5(2017)
• Page Start: 232
• Page End: 233
• Publication Date: 2017-05
• Subjects: rat -- fetal -- sex determination; Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000114548
• Languages: English
• ISSNs: 0736-6205

Abstract

The advent of genome editing strategies has expanded the range of animal models available for gene manipulation and renewed research interest in the rat. Gender is a key variable for in vivo gene function analyses. Here, we present a simple PCR-based method to determine genetic sex in the rat.