Comparison of Two PCR Techniques Used in Amplification of Microdissected Plant Chromosomes from Rice and Wheat. (April 2000)

• Record Type: Journal Article
• Authors: Zhou, Y.; Wang, H.; Wei, J.; Cui, L.; Deng, X.; Wang, X.; Chen, Z.
• Is Part Of: Biotechniques. Volume 28:Number 4(2000)
• Page Start: 766
• Page End: 774
• Publication Date: 2000-04
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/00284rr06
• Languages: English
• ISSNs: 0736-6205

Abstract

Linker adaptor-mediated PCR (LAMPCR) and degenerate oligonucleotide primed PCR (DOP-PCR) are major ways to generate chromosome-specific libraries. In this study, these two PCR techniques were used to amplify the microdissected mitotic and meiotic chromosomes from rice and wheat. The results of amplifications were compared, and the advantages and limitations between the two techniques are presented.