Detection of Eukaryotic cDNA in Differential Display Is Enhanced by the Addition of E. coli RNA. (January 2000)

• Record Type: Journal Article
• Authors: Melichar, H.; Bosch, I.; Molnar, G.M.; Huang, L.; Pardee, A.B.
• Is Part Of: Biotechniques. Volume 28:Number 1(2000)
• Page Start: 76
• Page End: 82
• Publication Date: 2000-01
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/00281st01
• Languages: English
• ISSNs: 0736-6205

Abstract

We describe a method to enhance the sensitivity of eukaryotic cDNA detection in differential display (DD). Typically, DD protocols require between 200 and 500 ng RNA for each reverse transcription reaction. The addition of Escherichia coli RNA before reverse transcription of eukaryotic RNA increases the detection of DD patterns more than tenfold. The method broadens the applicability of DD and allows the identification of genes that are differentially expressed when the amount of eukaryotic RNA is limited.