Use of Enhanced Green Fluorescent Protein to Optimize and Quantitate Infection of Target Cells with Recombinant Retroviruses. (May 1999)

• Record Type: Journal Article
• Authors: Cashion, L.M.; Bare, L.A.; Harvey, S.; Trinh, Q.; Zhu, Y.; Devlin, J.J.
• Is Part Of: Biotechniques. Volume 26:Number 5(1999)
• Page Start: 924
• Page End: 930
• Publication Date: 1999-05
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/99265rr02
• Languages: English
• ISSNs: 0736-6205

Abstract

Recombinant retroviral vectors are useful tools for gene transfer in both gene therapy and research applications. An enhanced form of green fluorescent protein has been incorporated into recombinant retroviruses as a marker to follow infected cells. In this paper, we extended the use of the fluorescent reporter to quantify protein expression using such analytical tools as fluorescent microscopy, flow cytometry and fluorescent plate reader analysis. These tools enabled us to rapidly assess the titer of recombinant retrovirus harvested from packaging cells and to optimize parameters for infection of different cell lines.