The Primer Generator: A Program that Facilitates the Selection of Oligonucleotides for Site-Directed Mutagenesis. (April 1999)

• Record Type: Journal Article
• Authors: Turchin, Alexander; Lawler, Joseph F.
• Is Part Of: Biotechniques. Volume 26:Number 4(1999)
• Page Start: 672
• Page End: 676
• Publication Date: 1999-04
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/99264st02
• Languages: English
• ISSNs: 0736-6205

Abstract

Site-directed mutagenesis is a powerful tool that has enabled molecular biologists to perform functional analysis of altered nucleic acids and proteins. Newer PCR-based mutagenesis techniques have reduced the process of mutagenesis to as little as one day. While each technique has its advantages, both require a strategy to isolate the desired clone from a population that contains mutagenized and wild-type genes. In this report, we describe a World Wide Web-based computer program that facilitates the design of mutagenic primers such that successfully mutagenized clones can be identified by the presence or absence of a unique restriction site.