Colorimetric Method for Detecting Amplified Nucleic Acids. (October 1998)

• Record Type: Journal Article
• Authors: Durward, Elaine; Harris, William J.
• Is Part Of: Biotechniques. Volume 25:Number 4(1998)
• Page Start: 608
• Page End: 614
• Publication Date: 1998-10
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/98254st01
• Languages: English
• ISSNs: 0736-6205

Abstract

Methods for detecting and measuring, the success of nucleic acid sequence amplifications, can be developed by detecting the by-products of amplification procedures. One method includes the detection of inorganic phosphate (Pi) during or on completion of the PCR. The method requires modification of assay conditions to prevent thermal- and template-independent enzymatic activity from nonspecifically hydrolyzing dNTPs. Detection of Pi by the traditional Fiske-SubbaRow method provides a sensitivity similar to ethidium bromide staining of amplified products. The method offers a simple and rapid assay for amplified nucleic acids and can be useful in assays where confirmation of the amplified DNA product is not essential.