Highly Efficient Oligonucleotide Transfer into Intact Yeast Cells Using Square-Wave Pulse Electroporation. (August 1998)

• Record Type: Journal Article
• Authors: Barre, François-Xavier; Mir, Lluis M.; Lécluse, Yann; Harel-Bellan, Annick
• Is Part Of: Biotechniques. Volume 25:Number 2(1998)
• Page Start: 294
• Page End: 296
• Publication Date: 1998-08
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/98252gt04
• Languages: English
• ISSNs: 0736-6205

Abstract

Here, we present a rapid and reproducible procedure based on square-wave pulse electroporation that allows efficient penetration of synthetic oligonucleotides into intact yeast cells. This procedure was successfully used to modify the yeast genome with small amounts of oligonucleotide.