Versatile Epitope Tagging Vector for Gene Expression in Mammalian Cells. (August 1998)

• Record Type: Journal Article
• Authors: Hosfield, Tanya; Lu, Quinn
• Is Part Of: Biotechniques. Volume 25:Number 2(1998)
• Page Start: 306
• Page End: 309
• Publication Date: 1998-08
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/98252pf01
• Languages: English
• ISSNs: 0736-6205

Abstract

We have constructed an epitope-tagging vector, pCMV-Tag 1, for gene expression in mammalian cells. This vector, which allows for N-terminal, C-terminal and internal tagging of the gene product of interest with the FLAG ® and/or c-myc epitopes, enables researchers to rapidly and efficiently characterize gene products in vivo.