Making Genes Green: Creating Green Fluorescent Protein (GFP) Fusions with Blunt-End PCR Products. (July 1998)

• Record Type: Journal Article
• Authors: Lo, Wayne; Rodgers, William; Hughes, Thomas
• Is Part Of: Biotechniques. Volume 25:Number 1(1998)
• Page Start: 94
• Page End: 98
• Publication Date: 1998-07
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/98251st05
• Languages: English
• ISSNs: 0736-6205

Abstract

The jellyfish green fluorescent protein (GFP) has proven to be a useful tool in protein localization and trafficking studies. Fused to GFP, a protein of interest can be visualized and tracked in vivo through fluorescence microscopy. However, the process of making these fusion proteins is often tedious and painstaking. Here, we describe a simple and quick method for creating GFP fusion proteins using blunt-end PCR product ligation.