Rainbow™ Universal CPG: A Versatile Solid Support for Oligonucleotide Synthesis. (April 1997)

• Record Type: Journal Article
• Authors: Nelson, P.S.; Muthini, S.; Vierra, M.; Acosta, L.; Smith, T.H.
• Is Part Of: Biotechniques. Volume 22:Number 4(1997)
• Page Start: 752
• Page End: 756
• Publication Date: 1997-04
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/97224pf01
• Languages: English
• ISSNs: 0736-6205

Abstract

We have developed a universal solid support, termed Rainbow™ Universal CPG, for use in automated oligonucleotide synthesis. The universal solid support allows any oligodeoxyribonucleotide sequence to be synthesized from a single type of controlled pore glass (CPG) support. Deprotection of oligodeoxyribonucleotides was optimized using 0.5 M LiCl in concentrated ammonium hydroxide. PCR experiments using three different sets of primers proved that the 3′-hydroxyl function of oligodeoxyribonucleotides synthesized from Rainbow Universal CPG was retained. This universal solid support shows promise for replacing the standard nucleoside CPG supports.