Determination of Human NAT2 Acetylator Genotype by Oligonucleotide Ligation Assay. (April 1997)

• Record Type: Journal Article
• Authors: Bigler, Jeannette; Chen, Chu; Potter, John D.
• Is Part Of: Biotechniques. Volume 22:Number 4(1997)
• Page Start: 682
• Page End: 690
• Publication Date: 1997-04
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/97224st03
• Languages: English
• ISSNs: 0736-6205

Abstract

The oligonucleotide ligation assay (OLA) was adapted to the genotyping of the N -arylamine-acetyltransferase ( NAT2 ) gene. This assay allows the use of 96-well microplates and robotic workstations for high sample throughput. We found this assay to be accurate, efficient and reliable. Another advantage for epidemiological studies where the DNA supply is limited is the small amount of genomic DNA required. A single PCR with an input of 50–100 ng of genomic DNA provides sufficient amounts of amplified NAT2 fragment to analyze five missense mutations.