Cell Surface Display of a Single-Chain Antibody for Attaching Polypeptides. (October 1996)

• Record Type: Journal Article
• Authors: Rode, H.-J.; Little, M.; Fuchs, P.; Dörsam, H.; Schooltink, H.; Inés, C. de; Dübel, S.; Breitling, F.
• Is Part Of: Biotechniques. Volume 21:Number 4(1996)
• Page Start: 650
• Page End: 658
• Publication Date: 1996-10
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/96214st02
• Languages: English
• ISSNs: 0736-6205

Abstract

To provide an efficient means of coupling proteins, peptides and other suitable moieties to cells, we have constructed a retroviral expression vector for cell surface display of a single-chain antibody (scFv) against the hapten 4-ethoxymethylene-2-phenyl-oxazoline-5-one (phOx). The hapten phOx can be easily conjugated to primary amino and sulfhydryl groups, thus providing points of attachment for the cell surface-bound antiphOx scFv. This universal cell coupling system could prove to be particularly useful for anchoring monoclonal antibodies for tumor targeting and to present co-stimulatory molecules and other ligands (even mixtures) at the cell surface for gene therapy.