Isolation of High-Molecular-Length DNA from Human Skin. (September 1996)

• Record Type: Journal Article
• Authors: Bennett, P.V.; Gange, R.W.; Hacham, H.; Hejmadi, V.S.; Moran, M.; Ray, S.; Sutherland, B.M.
• Is Part Of: Biotechniques. Volume 21:Number 3(1996)
• Page Start: 458
• Page End: 463
• Publication Date: 1996-09
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/96213st03
• Languages: English
• ISSNs: 0736-6205

Abstract

An agarose plug method for isolating high-molecular-length DNA from mammalian tissues has been developed, including from those that are difficult, such as skin. It gives high yields of DNA that contain a minimum of single-strand breaks and is readily digested by restriction and other nucleases. The method requires only simple equipment and is readily adaptable to field or clinical studies.