Measurement of Cell Adhesion and Migration Using Phosphor-Screen Autoradiography. (August 1996)

• Record Type: Journal Article
• Authors: Dalton, B.A.; Dziegielewski, M.; Johnson, G.; Underwood, P.A.; Steele, J.G.
• Is Part Of: Biotechniques. Volume 21:Number 2(1996)
• Page Start: 298
• Page End: 303
• Publication Date: 1996-08
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/96212rr04
• Languages: English
• ISSNs: 0736-6205

Abstract

This study demonstrates the use of phosphor-screen autoradiography as a means of measuring cell adhesion and cell expansion on polymer surfaces. The method has particular merit in cases where a specific substrate may be opaque or biochemically incompatible with colorimetric assay methodologies. With the phosphor-screen autoradiography method, there was a linear relationship between cell number and quantitated radioactivity. The technique has also been validated by comparison with a colorimetric assay of adhesion conducted for attachment to conventional culture substrata. The data supported the view that the use of phosphor-screen autoradiography was a valid method for detecting cell attachment, and it gave equivalent results to the colorimetric assay. Furthermore, a comparison between phosphor-screen autoradiography and a dye-staining method showed that this technique can be used as a means of quantifying cellular expansion over surfaces.