Precision 96-Channel Dispenser for Microchemical Techniques. (February 1996)

• Record Type: Journal Article
• Authors: Stanchfield, J.; Wright, D.; Hsu, S.; Lamsa, M.; Robbins, A.
• Is Part Of: Biotechniques. Volume 20:Number 2(1996)
• Page Start: 292
• Page End: 296
• Publication Date: 1996-02
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/96202pf01
• Languages: English
• ISSNs: 0736-6205

Abstract

A new automated 96-channel microdispenser is described for precise, high-speed dispensing of microliter volumes of reagents. The Hydra-96™ is a programmable instrument composed of 96 glass syringes arrayed in a microplate format that fills and dispenses in unison under computer control. Studies show the instrument has less than a 2% coefficient of variation (CV) across the syringe array when dispensing between 0.5 and 20.0 μL of reagent. Blot hybridization studies demonstrate a simple rinsing protocol using 2% bleach that efficiently cleans the system of DNA without affecting subsequent PCRs. Current uses of the instrument in assembling microassays used in large-scale genetic mapping and sequencing projects and compound library screening are discussed.