MicroRNA-509–3p increases the sensitivity of epithelial ovarian cancer cells to cisplatin-induced apoptosis. (February 2016)

• Record Type: Journal Article
• Authors: Chen, Wei; Zeng, Wenshu; Li, Xiaodi; Xiong, Weiliang; Zhang, Mengdie; Huang, Yan; Zhou, Longshu; Jiang, Songshan
• Is Part Of: Pharmacogenomics. Volume 17:Number 3(2016)
• Page Start: 187
• Page End: 197
• Publication Date: 2016-02
• Subjects: cell apoptosis -- cell proliferation -- microRNA-509–3p -- ovarian cancer -- X-linked inhibitor of apoptosis; Pharmacogenomics -- Periodicals; 615.1
• DOI: 10.2217/pgs.15.166
• Languages: English
• ISSNs: 1462-2416

Abstract

Aims: XIAP is upregulated in chemoresistant epithelial ovarian cancer (EOC). However, the molecular mechanism of this dysregulation remains unclear.Materials & methods: The regulation of XIAP by miR-509-3p was investigated by luciferase reporter assay, real-time PCR and immunobloting, and the roles of miR-509-3p in cellular proliferation and apoptosis were accessed through MTT and DAPI assays.Results: miR-509-3p, a downregulated miRNA in chemoresistant EOC, can directly target the XIAP via its 3′UTR. Overexpression of miR-509-3p can not only downregulate the expression of XIAP in ovarian cancer cells but also inhibit the proliferation of EOC cells and increase their sensitivity to cisplatin-induced apoptosis.Conclusions: Our data suggest that restoring certain dysregulated miRNAs to their normal levels could increase the therapeutic effects of anticancer drugs.